Multiplex nested RT-PCR for detecting avian influenza virus, infectious bronchitis virus and Newcastle disease virus
TT Nguyen, HJ Kwon, IH Kim, SM Hong… - Journal of Virological …, 2013 - Elsevier
In this study, multiplex nested RT-PCR (mnRT-PCR) was applied to simultaneous detect
multiplex PCR with the higher sensitivity of nested PCR that is required for avian influenza,
infectious bronchitis and Newcastle disease virus using two steps of amplification. For the
first PCR, primers that were specific for each virus were newly designed from the
nucleoprotein gene of AIV, the nucleocapsid protein gene of IBV and the fusion protein gene
of NDV to amplify products of 665, 386 and 236 nucleotides, respectively. The multiplex …
multiplex PCR with the higher sensitivity of nested PCR that is required for avian influenza,
infectious bronchitis and Newcastle disease virus using two steps of amplification. For the
first PCR, primers that were specific for each virus were newly designed from the
nucleoprotein gene of AIV, the nucleocapsid protein gene of IBV and the fusion protein gene
of NDV to amplify products of 665, 386 and 236 nucleotides, respectively. The multiplex …
Multiplex nested RT-PCR for detecting avian influenza virus, infectious bronchitis virus and Newcastle disease virus.
TTN Thanh Trung Nguyen, KHJ Kwon HyukJoon… - 2013 - cabidigitallibrary.org
In this study, multiplex nested RT-PCR (mnRT-PCR) was applied to simultaneous detect
multiplex PCR with the higher sensitivity of nested PCR that is required for avian influenza,
infectious bronchitis and Newcastle disease virus using two steps of amplification. For the
first PCR, primers that were specific for each virus were newly designed from the
nucleoprotein gene of AIV, the nucleocapsid protein gene of IBV and the fusion protein gene
of NDV to amplify products of 665, 386 and 236 nucleotides, respectively. The multiplex …
multiplex PCR with the higher sensitivity of nested PCR that is required for avian influenza,
infectious bronchitis and Newcastle disease virus using two steps of amplification. For the
first PCR, primers that were specific for each virus were newly designed from the
nucleoprotein gene of AIV, the nucleocapsid protein gene of IBV and the fusion protein gene
of NDV to amplify products of 665, 386 and 236 nucleotides, respectively. The multiplex …
